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Literature summary extracted from

  • Diekmann, L.; Behrendt, M.; Amiri, M.; Naim, H.Y.
    Structural determinants for transport of lactase phlorizin-hydrolase in the early secretory pathway as a multi-domain membrane glycoprotein (2017), Biochim. Biophys. Acta, 1861, 3119-3128 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.4 expressed in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.108 additional information the enzyme without domain II (LPHDELTA2) is normally transported along the secretory pathway, but does not dimerize nor is enzymatically active Homo sapiens
3.7.1.4 N1340Q the variant lacks the closest potential N-glycosylation site to Asp1338 Homo sapiens
3.7.1.4 N1814Q the variant lacks the N-glycosylation site in the domain IV Homo sapiens
3.7.1.4 N821Q the variant lacks the N-glycosylation site in the domain II Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.108 endoplasmic reticulum
-
 Homo sapiens 5783
-
3.2.1.108 membrane
-
 Homo sapiens 16020
-
3.7.1.4 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.4 250000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.108 Homo sapiens P09848
-
-
3.7.1.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.108 glycoprotein the polypeptide stretch in domain II between L735-R868 harbors a unique N-glycosylation site that is responsible for lactase phlorizin-hydrolase retention in the endoplasmic reticulum via association with calnexin and facilitates proper folding of domains I and III before ER exit of lactase phlorizin-hydrolase. A similar N-glycosylation site in domain IV shows comparable effects on the trafficking of lactase phlorizin-hydrolase-derived molecules Homo sapiens
3.7.1.4 glycoprotein
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.108 intestinal epithelium
-
 Homo sapiens
-
3.7.1.4 intestinal epithelium
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.4 lactose + H2O
-
 Homo sapiens D-galactose + D-glucose
-
 ?
3.7.1.4 phlorhizin + H2O
-
 Homo sapiens 3-(4-hydroxyphenyl)propanoate + arbutin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.4 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.108 lactase phlorizin-hydrolase
-
 Homo sapiens
3.7.1.4 lactase phlorizin-hydrolase
-
 Homo sapiens
3.7.1.4 LPH
-
 Homo sapiens